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Abstract In order to study gene expression profile during appressorium developmental process of Magnaphorthe grisea strain Y34 that isolated from the rich area for Asia cultivated rice resources, a cDNA array with 4108 TUTs was used to monitor gene expression patterns during appressorium development of M. grisea. A total of 511 differentially expressed genes were identified from 0, 2, 8, 20, and 30 h time points of the infection structure development. Compared with ungerminated conidia, the number of up-regulated and down-regulated genes was almost consistent at any time-points. More genes were differentially expressed during appressorium maturation than during appressorium induction and formation. Gene expression profiles between 8 and 20 h time-points were similar. By 30 h time-point, all genes that were down-regulated in conidia had changed to up-regulated, and half of the genes showing up-regulated in conidia changed to down-regulated. During 20 to 30 h time-points, genes seemed to be most actively expressed. Therefore, a detailed and comprehensive analysis of the programmed gene expression that occurs during the continuous stages of appressorium development is necessary to better understand the pathogenesis of M. grisea.
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Received: 15 March 2007
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