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Abstract The head kidney macrophages and peripheral blood granulocytes isolated from the head kidney of Cyprinus carpio were purified by means of Percoll gradient density centrifugation and were exposed to Lentinan in vitro. The effects of the polysaccharide on the peripheral blood granulocytes proliferation, respiratory burst of macrophage and IL-1βmRNA expression of macrophages were investigated by using MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) ,NBT(nitroblue tetrazolium) reduction, Griess reagent coloration and Real time PCR respectively, to evaluate the immunostimulant of the polysaccharide and compare its immune activities with the control group. Results showed that Lentinan stimulated markedly the proliferation of the peripheral blood granulocytes and could induce superoxide anion in macrophages markedly. Lentinan had no conspicuous effect on nitrogen burst activity of macrophages, moreover it inhibited the nitrogen burst activity at higher doses. The polysaccharide stimulated IL-1βexpression in carp head kidney . It is suggested that Lentinan could modulate the immune response of Cyprinus carpio.
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Received: 21 November 2007
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