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| Optimization of SRAP-PCR system with orthogonal design in Chinese date |
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Abstract The optimal SRAP-PCR system in Chinese date was established with orthogonal design. In a total volume of 20µL SRAP-PCR system, it contains1×buffer, 200µmol/L dNTP, 30ng Primer, 2.5mmol/L MgCl2, 1.0U TaqDNA polymerase and 20ng template DNA. The suitable PCR procedure was initially denaturing at 94℃ for 5min; then 94℃,1min, 33℃,lmin, and 72℃,1min for the first five cycles; then the annealing temperature is raised to 53℃ for another 30 cycles. The new established SRAP-PCR system of Chinese date was fully reproducible and good stability.
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Received: 31 January 2007
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