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Abstract Investigate the capability of spermatogonial stem cells(SSCs) differentiating into adipocytes under different experimental conditions in vitro. In present study, SSCs were isolated from chicken embryo testis on 16th hatching days, then cultured and subcultured in vitro. SSCs were induced into adipocytes in medium containing desamethason, insulin and IBMX. The differentiated cells were then identified by oil red-O staining. Results showed that SSCs were induced into adipocytes during 7～21d after cultured in induction medium. The programmer ⑥ showed the best result with 85% oil red-O staining positive SSCs.The induced adipocytes highly expressed PPARγ. The cooperative inductive effect was higher significantly than alone inductive effect of desamethason, insulin and IBMX（p<0.01）. Therefore, we concluded that Chicken embryonic SSCs have the capability of multipotential differentiation into adipocytes in vitro.
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Received: 27 August 2007
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