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| Research about the Expression of modified VHb Gene and fusedly- insectidal Gene in Tobacco |
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Abstract The highly-efficient plant binary expression vector PGBIF4ABCVHB was constructed by applying the artificially-modified-synthesized VHb gene and fusedly-insectidal gene(GFMcryIA and CPTI ) , 38 transgenic tobacco plants were obtained by Agrobaterium mediated transformation ,32 of them were indentified that the bivalent genes were inserted into the tobacco genome by PCR amplification detection and Southern blot analysis,western blot analysis proved that VHb gene was expressed in the transgenic plants,Toxicity assay indicated that fused insectidal gene was expressed pesticadal toxin protein .the net weight of transgenic tobacco plants exceeded 8% of that of non- transgenic ones . compared to non- transgenic tobacco plants, the transgenic ones were high-yield varities of insect-resistant plants.
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Received: 10 January 2007
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