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| SNP in intron 5 of HMGR Gene and its genetic effects on important economic trait in goose |
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Abstract The intron 5 of the 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) gene was cloned and its SNP was tested by PCR-SSCP in Wanxi White goose (WW). The carcass trait, meat quality trait and feather-down trait of 116 WWs were mensurated and analyzed by relating with SNP. The results showed that intron 5 sequence length of HMGR gene in goose was 704bp, and the homology between goose and chicken was 44.0%. A and B alleles were tested in the intron 5 by PCR-SSCP, and gene frequencies of A and B were 0.7716 and 0.2284 respectively. Genotype AA was dominant genotype on increasing chest muscle weight, gland stomach weight (P<0.05) and 1000-downs weight (P<0.01), and on decreasing down hold-water percentage (P<0.05). Genotype AB was dominant genotype on decreasing fiber diameter of chest muscle (P<0.01) and on increasing chest muscle 45pH (P<0.05).
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Received: 06 May 2007
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