Abstract 【OBJECTIVE】 to establish model of piglet testis tissue in vitro spermatogenesis;【METHOD】Using 5＇-Bromo-2＇- deoxyuridine（BrdU）immunofluorescence technique to detect the proliferation and differentiation of germ cells from testis tissue..And,in this research the Hematoxylin-eosin staining(HE) was also used to identify the differentiation degree ；【RESULTS】As a result,we found that on Day 3 of in vitro culture,sertoli cells began to adhere to the dish;on Day 4~8 of in vitro culture,the germ cells began to travel from the tissue, yet no proliferation or differentiation can be seen；on Day 9~20 of vitro culture , proliferation or differentiation can be clearly observed ,germ cells were linked by the gap junction presented as grape cluster or the strings of beads, and the most exiting thing was that we also found the spermatid in the dish;【CONCLUSION】germ cells can polify or differentiate in this system,and the most interesting thing is that germ cells can differentiate into spermatids without addition of testosterone.
