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Abstract Abstract: To express the Staphylococcal α-Hemolysin gene in E. coli and study its primary biological characterristics, α-Hemolysin (α-HL) gene without signal peptide sequence was amplified from Staphylococcus aureus by PCR and inserted into pMD18-T vector. The cloned α-HL gene was then inserted into prokaryotic expression vector pET32a+ and transformed into E. coli BL21. The predicted protein was detected by SDS-PAGE after IPTG induction, which had molecular weight approximately 53 kD. Hemolytic experiment demonstrated the expressed protein can lyse mouse red cell and its hemolytic titer attained 2.26 ×104 HU/mg. Conclusively, the Staphylococcal α-HL gene was successfully cloned and expressed in E. coli. The successful expression of α-HL in E. coli BL21 constituted a solid foundation for further researches such as pathogenesis and immune mechanism and vaccine development.
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Received: 21 August 2006
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