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Abstract Oxygen tension and culture media (CM) as well as growth factors are the key factors to the development of embryos in vitro. Systematically study had been carried on effect of oxygen tension（7% O2和20% O2）, culture media (NCSU-23、 PZM-3、G3) and growth factors(EGF, bFGF）on in vitro development of porcine parthenogenetically activated(PA) embryos, including blastocyst formation rate and cell number per blastocyst. Compared with 20%O2, low oxygen atmosphere improved blastocyst rate and total cell number; PA embryos in PZM-3 and 7 %O2 had a greater cell number (P < 0.05, 51.0±4.0 vs. 38.8±2.2). Regardless of atmosphere, PZM-3 supported a higher development rate and quality of porcine PA embryos than NCSU23 respectively. 2-4 cell stage PA embryos cultured in NCSU-23 supplemented with 10ng/mL EGF or bFGF had a significantly greater developmental ability to blastocyst than the control （P < 0.05, 36.2±5.3％，44.3±5.8％ vs. 20.7±6.3％）, no difference at blastocyst cell number between them.
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Received: 20 November 2006
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