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| Cloning and the Difference Expression of Porcine SOCS3 Gene at Different Stages |
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Abstract Genome RNA was extracted from mesentery adipose of porcine (Duroc×Landrace×Yorkshire) and suppressor of cytokine signaling -3(SOCS3) mRNA was amplified using RT-PCR. A DNA fragment about 332 bp in length was obtained and the PCR product was cloned into pGEM-T vector. SOCS3 gene was isolated and sequenced from the positive clones screened. Sequence analysis suggested that this fragment corresponded to a portion of the sequence of SOCS3 cDNA, its homology with SOCS3 from GenBank was up to 100%. Based on the SOCS3 gene clone, an optimal semi-quantitative RT-PCR method was successfully constructed. Using 18s rRNA as inner control, the difference of SOCS3 gene expression at different stages of swine was studied. The results showed that the amount of SOCS3 gene expression increased from 1 kg to 90 kg. and the SOCS3 mRNA level at 90 kg significantly increased by 141% (p<0.05) as compared with 1 kg.
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Received: 10 November 2006
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