|
Abstract Inverted repeat plant expression vector pKcp was constructed with peanut stripe virus(PStV) coat protein gene, then transformed into Agrobacterium tumefaciens strain GV3101. Transgenic plants were obtained by transformation of Nicotiana benthamiana leaf discs by agrobacteirum and kanamycin screening. The transgenic plants were tested for its resistance by inoculation with PStV in 5~6 leaf stage. By symptom observation and ELISA detection, there was 87% transgenic plants immune to PStV in T0 , 60~100% immune to PStV in T1 different lines, most of T2 lines immune to PStV. siRNA was detected by Northern blot by PStV cp probe in T1 plants, different amount of siRNA was detected in transgenic plants, no siRNA was found in nontransgenic plants; siRNA amount was higher in resistant plants than susceptible plants in the leaves before inoculation; about same amount of siRNA of leaves were detected in different days post inoculation in the same resistant plant, different amount of siRNA was found in different resistant plants. In this experiment, dsRNA strategy were succeeded inducing high frequency of transgenic resistant tobacco plants to PStV.
|
|
Received: 29 December 2006
|
|
|
|
