|
Abstract For mapping and characterizing QTLs related to Phytophthora capsici (PC) resistance in pepper, a molecular linkage map was constructed with AFLP markers in an intraspecific F2 generation population by crossing 93-100-17-1-0, a highly resistant to PC, with Qiemen, an inbred line susceptible to PC. A total of 165 F2 generation plants from the cross between 93-100-17-1-0 and Qiemen were used for identification of DNA markers linked to PC resistance. The results of the genetic statistical analysis using three different criteria, receptivity, inductivity, and stability, indicated that the resistance of 93-100-17-1-0 to PC was controlled by polygenes or oligogenes. A genetic map was constructed using the software Mapmaker/exp, which contained 11 linkage groups with 46 linked molecular markers and covered 1381.5 cM of the whole genome. Using the three different criteria as traits, 18 QTLs localizing on LG1, LG2, LG5, LG7 and LG8, which explained over 64% of the phenotypic variance, were identified using the Function Interval mapping and Composite Interval mapping of the software winQTLCART. The most significant QTL localized on LG5 from 128 cM to 132 cM, which explained 68% of the phenotypic variance and whose genetic distance was only 4 cM.
|
Received: 01 February 2007
|
|
|
|
|
|