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Abstract Abstract: The human erythropoietin gene (hEPO), containing all exons and introns of the hEPO gene except intron 1, was cloned by using PCR technique and synthesized. The hEPO gene was subcloned into the pIRES2-AcGFP1 to construct the eukaryotic expression vector to co-expression hEPO and AcGFP1. The vector was transfected into Chinese hamster ovary cells (CHO) and goat fibroblast by lipidosome transfection. The results showed that CHO and goat fibroblasts appeared bright fluorescence 48h after transfection. Screened by G418, expression cells were obtained. This indicated that the eukaryotic expression vector of hEPO gene with reporter gene has been constructed and expressed in CHO cells and goat fibroblasts. The results paved the way for detecting whether hEPO gene had been transduced into cells, as well as screening positive cells.
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Received: 30 July 2006
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