Abstract Several Phytophthora infestans-susceptible mutants had been obtained based on the different reactions of Arabidopsis activated tagging mutants after being inoculated by the fungus, and flanking sequence of Arabidopsis T-DNA insertion had also been obtained by TAIL-PCR technology and genomic DNA from disease-susceptible mutants as template. Some disease resistant-related genes had been found using NCBI sequence homology analysis and AD1,AD2 and AD4 were the most efficient arbitary primers. Two mutants had been found with T-DNA insertion through sequencing and analyzing eight specific TAIL-PCR products.