|
|
|
| Analysis T-DNA flanking sequence of Arabidopsis thaliana mutants of resistant to Phytophthora infestans |
| |
| |
|
|
|
|
Abstract Several Phytophthora infestans-susceptible mutants had been obtained based on the different reactions of Arabidopsis activated tagging mutants after being inoculated by the fungus, and flanking sequence of Arabidopsis T-DNA insertion had also been obtained by TAIL-PCR technology and genomic DNA from disease-susceptible mutants as template. Some disease resistant-related genes had been found using NCBI sequence homology analysis and AD1,AD2 and AD4 were the most efficient arbitary primers. Two mutants had been found with T-DNA insertion through sequencing and analyzing eight specific TAIL-PCR products.
|
|
Received: 26 October 2006
|
|
|
|
|
|
|
