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Analysis T-DNA flanking sequence of Arabidopsis thaliana mutants of resistant to Phytophthora infestans
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Abstract  Several Phytophthora infestans-susceptible mutants had been obtained based on the different reactions of Arabidopsis activated tagging mutants after being inoculated by the fungus, and flanking sequence of Arabidopsis T-DNA insertion had also been obtained by TAIL-PCR technology and genomic DNA from disease-susceptible mutants as template. Some disease resistant-related genes had been found using NCBI sequence homology analysis and AD1,AD2 and AD4 were the most efficient arbitary primers. Two mutants had been found with T-DNA insertion through sequencing and analyzing eight specific TAIL-PCR products.
Key wordsArabidopsis thaliana      Phytophthora infestans      activating tagging      TAIL-PCR     
Received: 26 October 2006     
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http://journal05.magtech.org.cn/Jwk_ny/EN/      OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2007/V15/I6/0
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