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| Molecular Characterization of SRK Kinase-domain from Brassica oleracea L. and Assay of It’s Interaction with THL1 |
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Abstract The DNA and cDNA fragments of SRK kinase domain(named as SRKE1) was amplified from stigma mRNA of Brassica oleracea L. by PCR and RT-PCR. Their lengths were 1241 bp and 1711 bp respectively. Sequence analysis indicated that the SRKE1 contained six exons and five introns，the SRKE1 encoded 407 amino acids. The coding sequence of SRKE1 were inserted into vector pET43.1 and pPIC9K to be the recombinant plasmid pET43.1-SRKE1 and pPIC9K-SRKE1. The recombinant protein were expressed in E. col(BL21) and Pichia pastoris GS115 repectively. When the SRKE1 was incubated with THL1 which we had expressed, SRKE1 could bind with THL1 and formed a stable complex.
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Received: 26 December 2007
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