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| The construction of plant expression vectors with PMI gene as selection marker and utilization in transformation of ‘Xuegan’ |
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Abstract The plant expression vectors pCAMBIA1301PMI and pBIPMI were constructed by substituting Escherichia coli phosphomannose- isomerase (PMI) gene for hpt gene of pCAMBIA1301 and GUS gene of pBI121. Epicocyl explants of ‘Xuegan’ sweet orange were inoculated with EHA105- pCAMBIA1301PMI and EHA105- pBIPMI and subsequently selected on medium supplemented with a combination of 25 g•L-1 mannose and 5 g•L-1 sucrose as a carbon source. The transformation efficiency rate was 27.7% when transformed by pCAMBIA1301PMI and 12.7% by pBIPMI .Genetic transformation was confirmed by Chlorophenol Red assay and PCR. A new method for obtaining transgenic ‘Xuegan’ plants was developed using PMI/mannose selection system.
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Received: 27 September 2007
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HUANG Xing, YAN Ai-Fen, DENG Ting-Xian, OUYANG Hong-Jia, LIU Lian, FENG Juan, ZHU Xiang-Xing, NIE Qing-Hua, TANG Dong-Sheng, ZHANG Xi-Quan. Construction of Zinc Finger Nuclease-induced Targeting Vector of Luchuan Pig (Sus scrofa) Fat1 Gene and Transgenic Study In vitro[J]. 农业生物技术学报, 2019, 27(8): 1369-1381. |
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