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| Medium Selection in Tissue Culture of Nipponbare Scutellum and Obtainment Regeneration of Transgenic Plants with Elicitor-coding Gene from Magnaporthe grisea |
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Abstract N6 medium, MS medium and BN medium were adopted in tissue culture of Nipponbare (Japanica) Scutellum for performance comparison. Results indicated that, among the three kinds of culture mediums, NB medium worked best in the calli induction and gene transformation and was most suitable for tissue culture of Nipponbare scutellum. Based on this, elicitor-encoding gene pemG1 from Magnaporthe grisea was introduced into the genome of Nipponbare via Agrobacterium-mediated method. Transgenic rice plants were obtained. The integration, transcription and expression of pemG1 in rice were respectively confirmed by PCR, Northern blot and Western blot. Genetic analysis showed that the transgenes were segregated normally in the progenies.
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Received: 17 January 2008
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HUANG Xing, YAN Ai-Fen, DENG Ting-Xian, OUYANG Hong-Jia, LIU Lian, FENG Juan, ZHU Xiang-Xing, NIE Qing-Hua, TANG Dong-Sheng, ZHANG Xi-Quan. Construction of Zinc Finger Nuclease-induced Targeting Vector of Luchuan Pig (Sus scrofa) Fat1 Gene and Transgenic Study In vitro[J]. 农业生物技术学报, 2019, 27(8): 1369-1381. |
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