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Abstract In vitro mature culture, collection with isolation and preservation of first polar bodies (PbΙ) in porcine oocytes were introduced in this study in order to supply some essential reference for recombination of porcine oocytes by the nucleis of PbΙ. Mature oocytes were obtained by in vitro culture of the follicle oocytes in ovary from abattoir, Expulsion, activity and morph of PbΙ were statistically analyzed. PbΙ was isolated and Collected by two methods of enzyme digestion or micrergy, their survival vigor under the different temperature preservation were approached and evaluated by Trypan blue staining method. The results showed that lots of high vigor PbΙ could be found by in vitro mature culture after 40 hours. The effect on isolation of PbΙ by micrergy outstripped enzyme digestion significantly, the isolated PbΙ by micregy existed ideal vigor and normal morph. Most of PbΙ survived during 4 hours under 39 ℃, rate of survivors was 85.0 % during 40 hours under 4 ℃, and over 95.0 % during a week under -20 ℃. The effect of cryopreservation was best for PbΙ, the rates of survivors and their normal morph were as high as 89.1% and 97.8%, respectively.
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Received: 12 November 2007
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