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Abstract Abstract: 【Objective】Using Real time PCR method detect three functional bacterias’ 16S rDNA copies in rumen liquid such as Anaerovibrio lipolytica, Fibrobacter succinogene, Ruminococcus flavefaciens, and building the method to reflect these bacteria quantities.【Method】At the same diet conditions ,collecting the rumen liquid microbe of different cows at the same time and the same cow at the different time. And extracting their total DNA. Design the special primers according to 16S rDNA. Then using real time PCR detect the different quantities of three functional bacteria.【Result】Three functional bacteria quantities at the same time have little different, however, there are obviously different about the same cow at different time point (P＜0.05). The main reason perhaps were the error on progress or method of collecting rumen bacteria samples.
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Received: 12 December 2007
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Corresponding Authors:
Dan Li
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