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| Association of Single Nucleotide Polymorphisms in Intron 1 of Porcine HNMMLC2B Gene with Meat Quality and Carcass Traits |
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Abstract Fast skeletal myosin light chain 2 (HUMMLC2B or MYLPF) is a Ca-binding protein which participate in many vital movements, and it has become a hot spot in the myosin studies in recent years．Using PCR-RFLP，the distributes of MspⅠ polymorphisms (T613C) in intron 1 of HUMMLC2B gene in seven pig populations were analyzed，and the relationship between the HUMMLC2B genotypes and pork quality and carcass traits was evaluated．The results showed the frequency of allele B were predominant in all other breeds except for Landrace, in which the ratio pf allele A and B was 1:2 and the homozygote of allele A was found. In six detected pig populations, very significant effects of this locus on loin eye width, loin eye area, intramuscular and water moisture (P<0.01) were found, and this locus was significantly associated with lean meat percentage and skin percentage (P<0.05). The expression of HUMMLC2B in the tissues also was examined. It needs further studied whether the polymorphic site is to be used as the candidate locus affecting carcass and meat quality traits in markers assisted selection.
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Received: 14 January 2008
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