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Abstract The GST-pMGA was expressed in E. coli BL21 with the pGEX fusion expression system. GST-pMGA expressed in supernatant was purified using GST·Bind Resin and a plenty of soluble protein was obtained, Overnight incubate GST-pMGA with thrombin protease, the pMGA was obtained and that showed well immunologic competency by Western-blot analysis. Indirect immunofluorescent assay was used to test interaction between pMGA and the tissue paraffin sections of trachea, heart, lung, liver, kidney, thymus, bursa of fabricius, spleen, proventriculus and the duodenum of SPF chick embryo; The results showed that pMGA and the tissues of chick embryo had a specific binding except the spleen, this binding indicated receptor of pMGA on the tissues of chick embryo. This will supply a foundation for the further study in receptor of pMGA.
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Received: 26 February 2008
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Corresponding Authors:
Xiu-Li Peng
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