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| Expression and Ferric Reductase Activity of Ferric Reductase Gene MxFRO from Malus xiaojinensis in Transgenic yeast |
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Abstract Malus xiaojinensis is iron-efficient genotype in the genus Malus. We isolated and cloned full-length cDNA sequence of MxFRO from Malus xiaojinensis , Semiquantitative RT-PCR was conducted, and MxFRO was found to be induced by iron-deficiency stress in roots and leaves. Yeast was transformed with MxFRO gene, and the ferric reductase activity in transgenic yeast was 2.8-fold that of controls.We concluded that MxFRO possessed ferric reductase activity.
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Received: 25 April 2007
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