|
Abstract Genetic diversity among 21 strains of Ralstonia solanacearum from different climate zones and different host crop was analyzed by ITS sequence and RAMS methods respectively. Three types of ITS sequence were obtained. Among them, there was only one or two base diversity, and ITS sequence of tested strains was the same to GMI1000 or one or two base difference to GMI1000.The total genomic DNA was then examined by sensitive RAMS technique. The results revealed an obvious diversity of their genomic DNA. Cluster analysis based on the RAMS bands showed that tested isolates could be separated into 3 clusters.The strains come from the same host crop were clustered into one group, and the strains come from the same climate zones were belonged to the same subgroup.It is concluded that genetic diversity of R.solanacearum comes from different host crop and different climate zones, mostly comes from different host crop.
|
|
Received: 28 November 2007
|
|
|
|
