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| Optimizing the expression of the subunit fasG of 987P fimbriae of Enterotoxigenic Escherichia coli（ETEC） |
| He-Feng LUO Jie WANG Zhong-Yong GOU Jian PENG |
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Abstract In our experiment, two E. coli expression vectors(pET28a(+)、pGEX-KG) with different translation intiation regions(TIR) and two E. coli expression strains(BL21(DE3)、Rossetta(DE3)) with different tRNA profiles had been used to obtain four bioengineered bacterium of fasG gene. The aim of the experiment was to examine the influence of secondary structures of TIR and the rare codens to the expression of fasG gene. The result of inducing expression of the bioengineered bacteriums showed that the secondary structures of TIR and the existence of rare codens can both influence the expression of target genes, optimizing either could both enhance the expression lever of target gene. The plasmid pKG-fasG had optimized the secondary structures of TIR and Rossetta(DE3) had overcome the rare condons’effects, so the expression lever of pKG-fasG Rossetta(DE3) achieved 16%, add to 100% in comparison with pET-fasG BL21(DE3)(8%). The result of this experiment indicated that the selection of appropriate expression vectors and strains was very important in the expressions of heterologous genes.
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Received: 07 November 2007
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Corresponding Authors:
He-Feng LUO
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