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| Increasement of Maize Amylose Content through RNA Interference |
| Xin-mei GUO |
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Abstract To regulate the biosynthesis of maize amylose, an expression vector of RNAi with the fragment of sense gene +FAD2 intron +antisense gene had been constructed, and. was transformed to maize calli which were induced to form from the elite inbred lines by Biolistic PDS1000/He. The selection was carried out on the media containing concentrations of xylose. 12 transformed plantlets were obtained. The integration of alien gene was conformed in 6 transformed plantlets with the analysis of DNA dot blotting, PCR and PCR-Southern hybridization. The seeds amylose content of regeneration plants was detected，and amylose content of a transgenic line increased up to 15.6%.
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Received: 06 November 2007
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Corresponding Authors:
Xin-mei GUO
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