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Abstract It has important practical significance to isolate and screen antagonistic actinomycetes from marine shrimp shell and study their antimicrobial activity. Streptomyces luteoverticillatus strain T0907-107 was isolated and identified from shrimp shell. In order to furtherly exploit and utilize the bacteria, antimicrobial activity substance was extracted from the thalli of strain T0907-107 which was collected from fermentation broth by 5 different solvents, acetone, ethyl acetate, n-butanol, ethanol and methanol. Antimicrobial activity and stability of the antimicrobial metabolites produced from strain T0907-107 were carried out. The results showed that acetone and methanol were the best solvents for the crude extract, and the inhibition zones were 26.20 and 22.50 mm, respectively. After protease K treatment, the crude extract still had inhibitory effects to the fungus. The crude extracted substance was material of thermostability (60 ℃) and had a good performance of acid and alkali resistance. The thalli of the strain and the crude extracted substance presented a strong antifungal activity against half of tested plant pathogenic fungi such as Colletotrichum nicotianae Sacc., Alternaria alternata(Fries)Keissler, Rhizoctonia solani Kühn, C. gloeosporioides (Penz.) Sacc. and C. higginsianum Sacc..The results showed that the acetone and methanol were good extraction solvents, the crude extracted substance had strong polarity and a strange of thermostability, acid and alkali resistance, which indicated that the bacteria had good development prospect. This study provides basic data for further identification of the active material and the exploitation and utilization of the strain T0907-107.
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Received: 17 July 2014
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