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Abstract PR domain containing 1, with ZNF domain(PRDM 1) is of great importance in the formation of primordial germ cell (PGC). A lot of reports focused on its function in the development of embryonic stem cell (ESC) to PGC, but it is unclear whether it has effects on the induction differentiation of adult stem cells or not. In this study, PRDM1 gene was cloned(GenBank No. JX154081.1) of mouse(Mus musculus), Lentivirus expression vector pCDH-PRDM1 was constructed and prepared Lentivirus particles using 293T cells, then, human umbilical cord mesenchymal stem cell (hUC-MSC) were transduced with PRDM1 gene-carrying virus. Results showed that PRDM1 could overexpress in hUC-MSC and upregulate the expression of some germ cell associated genes including stage-specific embryonic antigen-1(SSEA-1), developmental pluripotency associated 3 Dppa3(STELLA), stem cell growth factor receptor(C-KIT) and sex determining region Y-box 2(SOX2). The research provides important basis for further study of PRDM1, and is helpful to the research on induced differentiation of hUC-MSC towards germ cells.
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Received: 21 May 2014
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