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Abstract The porcine SMAF1 gene was cloned by degenerate PCR, PCR products for porcine SMAF1, generated from pocine adipose tissue, covered intact open reading frame which was identified with GeneScan software and via manual inspection. The length of this cDNA sequence was 256bp (GenBank DQ191892). The sequence for pig was similar to those of human(86%)and mouse(78％). The deduced amino acid sequence of porcine SMAF1 was similar to those of human(81%), mouse(67%), rat(70%) and bovine(84%).Semiquantitative RT-PCR analysis was performed to determine the expression pattern of SMAF1 in various types of porcine tissues and the genetic difference in mRNA concentrations of SMAF1 in two genetic populations of pigs ( Meishan and Large White). Transcripts for porcine SMAF1 were abundant in adipose tissue, we observed lower levels in Large White pigs than in Meishan pigs. The successful cloning and expression analysis of porcine SMAF1 will enhance the understanding of the involvement of
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Received: 15 May 2006
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