|
|
|
Abstract In order to efficiently select some advanced fragrant sterile lines by means of molecular breeding technology, the functional molecular marker 1F/1R of fragrance(fgr) gene was used to detect the polymorphisms of 13 maintainers of indica rice. Yixiang B was identified to haveⅠ- type band (fgr/fgr) (strong fragrance) by PCR strategy, and others had Ⅱ- type bands (Fgr/Fgr). 1F/1R was used to screen offsprings derived from a cross Ⅱ-32B / Yixiang B. The result suggested that 1F/1R showed codominance and was controlled by a single gene. By marker assisted selection(MAS) in the F2 generation, the homozygote rate of the marker 1F/1R in selected plants in F3 reached as high as 96.0%. Selecting the plants with fgr gene, together with the selection of traits in field and laboratory, such as plant type, grain type, transparency, chalk degree, gelatinization temperature, amylase content and so on, we obtained some plants interested among them and pairing mated with Ⅱ-32A. Through selection and pairing mating for 5 generations, with each generation into scale of about 50~60 pairs, we obtained some advanced fragrant sterile lines with stable traits, named Zhenongxiang A(B). Four representative sterile lines were selected to evaluate their comprehensive characteristics. These lines hadⅠ- type band at 1F/1R locus and they were considered as ideal advanced fragrant sterile lines with obvious fragrance, good plant types, slender grain type, high degree oftransparency, low degree of chalkiness, medium gelatinization temperature and amylase content. In conclusion, it is more effective to select the sterile lines of aromatic rice by functional marker fgr-1F/1R.
|
|
Received: 13 January 2011
|
|
|
|
|
|
