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Abstract Genetically modified corn(Zea mays) MON88017 is developed by U.S. Monsanto Company through recombinant DNA technology, which is characterized by resistances to maize beetles(Diabrotica virgifera) and to glyphosate herbicide. The purpose of this study is to develop and standardize the event-specific qualitative detection method of this line in China. Based on the information of the flanking sequences GM maize MON88017, a series of primer combinations were designed in the left margin of the binding site. The best primer pair was selected and the reaction system was optimized with the best primer pair. The maize MON88017 events-specific qualitative PCR detection method was established. After validated by 7 external laboratories, results showed that this method could specifically detect the samples of MON88017 event with the detection sensitivity about 0.1%, and had a good repeatability and reproducibility，thus suitable for detection of the corn MON88017 and a technical support for the implementation of the biosafety management regulations in China.
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Received: 08 March 2010
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