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| Construction and Partial Sequence Analysis of cDNA Library of Plutella xylostella |
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Abstract The diamondback moth (Plutella xylostella) is the grisly pests for cruciferous vegetables and crops. In this experiment, a cDNA library of the second instar larvae of diamondback moth was built by SMART (switching mechanism at 5' end of RNA transcript) technology. Sequencing and library analysis showed that the original storage capacity of the full-length cDNA library was 1.1×106, storage capacity of the amplified library was 2.6×109, the recombination frequency was 92%, with an average fragment size around 1 kb. All these indicated that the library quality met the requirements. Sequence analysis showed that the library contained some new genes, and some of them have been submitted in GenBank (Accession No. GW883877~GW883893, GU014922 ). Predicate the protein structure of the gene No. 11(GenBank accession No：GU014922) indicate that the gene belonged to gene family of serine protease, which was concerned with important metabolism and immunoreaction. The construction of the library may benefit for finding new gene and lay a foundation for the further genes function analysis of P. xylostella.
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Received: 20 February 2010
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