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Abstract Streptomyces venezuelae var. qinlingensis is a Zuelaemycin producting strain. For studying on effect of Vitreoscilla hemoglobin gene（vgb） expression in Streptomyces venezuelae var. qinlingensis and on Zuelacmycin production, it expressed through promoter of erythromycin resistance gene, characteristics of engineered strain and original strain growth and metabolism and Zuelaemycin valence were studied in 5 L fermenter. The result showed that: In high dissolved oxygen concentrations, the growth rate and ammonia nitrogen expenditure of engineered strain was slightly lower than that of the original strain, but utilization rate of starch was higher than that of the original strain, Zuelaemycin valence of engineered strain was decreased by 8% than that of original strain; in low dissolved oxygen concentrations, espression of hemoglobin enhanced growth, secondary metabolish and utilization rate of starch, and Zuelaemycin valence of engineered strain was more 45% than that of original strain.
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Received: 20 January 2010
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