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| Relative Quantification of mRNA Transcription of αv Subunits Relevant to Food and mouth disease virus (FMDV) Receptors in Different Tissues of Sheep by Real-time Quantitative RT-PCR |
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Abstract For establishing an assay of Real-time PCR to detect distribution and expression level of sheep Food and mouth disease virus (FMDV) integrin receptor αv submit in different tissue, in this study, a pair of primers was designed according to the published nudeotide sequence of integrin receptor αv submit. And tissue expression profile of sheep (Ovisaries) integrin αv in different tissue was detected by Real-time PCR. The results showed that the integrin receptor αv submit was generally expressed in 19 kinds of tissues with different level. The highest expression level was showed in breast, the next was in hoof, and the lowest was in muscle tissue. We have established the Real-time PCR method which can be used to confirm tissue profile of sheep FMDV integrin receptor αv submit. This study provides basic data for further study tissue tropism of FMDV and molecular detection method.
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Received: 29 September 2010
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