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| Cloning and Identification of Sunflower Seed-Specific Promoter Ha ds10 G1 |
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Abstract Abstract: The 1414bp 5’ flanking sequence of Ha ds10 G1 gene, a late embryogenesis abundant gene of Helianthus annuus L., was cloned by PCR. The similarity compared with the reported sequence was 100%. The promoter was fused to the glucuronidase gene_to construct plant expression vector, which was transferred into tobacco NC89 by Agrobacterium tumefaciens-mediated method. PCR results showed that the promoter had been integrated into genomic DNA of tobacco successfully. GUS activity assays indicated that expression of GUS was active only in transgenic tobacco seeds. However, the GUS activity didn’t exist in the stem and leaves. So, Ha ds10 G1 promoter is seed-specific.
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Received: 24 March 2006
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