|
|
|
| Isolation, Identification and Bioinformatics Analysis of Phosphatidy Lethanolamine-binding Protein 1 (PEBP1) Associated with Liver Toxicity Induced by Copper Nanoparticles in Rats (Rattus norvegicus) |
|
|
|
|
Abstract In order to screen the differentially expressed protein and explore the mechanism of hepatotoxicity induced by copper nanoparticles in rat(Rattus norvegicus), the differential proteins were separated and identified by two dimensional gel electro-phoresis (2-DE) and MALDI-TOF-TOF MS, and then verified by Real-time PCR, and analyzed by bioinformatics. Total 43 spots of differentially expressed proteins were found，of which a spot 1006 associated with hepatotoxity was identified as phosphatidy lethanolamine-binding protein 1(PEBP1)(accession No. IP1002309), the results of Real-time PCR was the same as that of 2-DE. Bioinformatics analysis showed that PEBP1 was located in the cytoplasm, and had no signal peptide, it may belongs to non-secretory protein, and contain phosphatidy lethanolamine-binding protein family signature 64YTLVLTDPDAPSRKDPKFREWHH86; the random coils and extended chains were its main secondary structural elements. The homology analysis showed that PEBP1 had a high homology between rat and other nine species, and the phylogenetic tree of PEBP1 was constructed by Neighbor-joint method. The expression of PEBP1 protein was reduced in poisoned rats so as to liver cell mitochondrial dysfunction, which may be a pathway of copper nanoparticles to exert the hepatotoxic effects.
|
|
Received: 20 June 2012
|
|
|
|
|
|
|
