|
|
|
| SNPs Detection of Transmembrane Protein 18 Gene(TMEM18) and Its Asscoation with Growth Traits in Jiaxian Red Cattle(Bos taurus) |
|
|
|
|
Abstract Transmembrane protein 18 gene(TMEM18) is expressed in the central nervous system that has recently been linked to human obesity and body mass index (BMI) in genome wide association studies (GWAS). In this study, direct DNA pool sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were used to genotype novel SNPs in TMEM18 gene, and then analyzed associations between different genotypes and growth traits in Jiaxian Red cattle(Bos taurus). Three novel SNPs were detected in Jiaxian Red cattle TMEM18 gene: NW_003099175.1: g. 2303G>A, g. 3835 G>A and g. 3865 A>G(aa. Gly>Ser). Significant association analysis revealed that the individuals with genotype AA had a larger body height, body length, heart girth and body weight than that of the individuals with genotypes GG at g.2303 A>G locus(P<0.05), The result also suggested that the individuals with genotype AG had a larger body weight and heart girth than that of the individuals with genotype AA at g. 3835 G>A locus (P<0.05). Therefore, these two loci can be used as body size and body weight candidate markers to breed Jiaxian Red cattles.
|
|
Received: 16 May 2012
|
|
|
|
|
|
|
