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| Cloning and Expression Characteristic of Trans-2,3-enoyl-CoA Reductase Gene(TaECR) in Physiological Male Sterile Line of Wheat (Triticum aestivum) |
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Abstract Very-long-chain fatty acids(VLCFAs) play an important role in anther development. VLCFAs is catalyzed by the fatty acyl-CoA elongase, a membrane-bound enzymatic complex containing trans-2,3-enoyl-CoA reductase(ECR). To further study the mechanism of male sterility induced by gametocide SQ-1 in wheat(Triticum aestivum L.), primers were designed based on silicon cloning sequence of ECR gene sequences from Brachypodium distachyon, and the open reading frame of the cDNA were obtained, here as designated TaECR that had been registered under GenBank(No. KC222053).Sequence analysis showed the open reading frame of this gene putatively encoding 310 amino acids, and had classical domains of ECR. Analysis showed that TaECR was ubiquitously expressed in anther, glume, leaf and root in wheat, but it was less expressed in root. Compared with fertile lines, the expression levels of TaECR in the physiological male sterile line were much lower than those in fertile line in trinucleate stage, but it showed no significant difference in unicleate and binucleate stage, which very corresponded to trends of wax loads in the development of the anther. These results suggest that the pathway of very long chain fatty synthetic metabolism regulated by TaECR gene may participate in abortion process of sterile line induced by SQ-1.
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Received: 18 October 2012
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