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| Detection of Actinobacillus pleuropneumoniae by newly developed loop-mediated isothermal amplification method |
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Abstract Here, we report a loop-mediated isothermal amplification (LAMP) assay with high sensitivity and specificity for the detection of Actinobacillus pleuropneumoniae. A set of six primers were designed, based on the ApxIV gene of A. pleuropneumoniae serotype 1. The detection limit of the LAMP assay completed within 45min at 63℃was 102 copies of the target sequence, which is 10 times more sensitive than the PCR assay. High species-specificity of the LAMP method were confirmed by the assay of 18 pathogens such as A. pleuropneumoniae serotypes 1~10, Pasteurella multocida, Streptococcus suis, Mycoplasma hyopneumoniae . In addition, All the nasal swab samples from 8 experimental infected pigs and 127 clinical cases were detected by LAMP assay, with a higher sensitivity than the PCR assay.
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Received: 10 April 2009
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NI He-Jia, CHENG Xue, WANG Jing, GAO Ze-Ping, WEI Pan-Pan, TAN Jia-Li, SUN Jing, LI Hai-Tao, GAO Ji-Guo. Isolation and Screening of High Virulent Bacillus thuringiensis Strains Against Lepidopteran Pests in Some Areas of Liaoning Province[J]. 农业生物技术学报, 2019, 27(8): 1513-1520. |
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