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| The quick screening SNP on partial coding sequence of porcine IGFBP5 gene and analysis of gene effect on production performance in pigs |
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Abstract Abstract: The insulin-like growth factors (IGFs) play a central role in cellar growth, division and differentiation, their binding proteins (IGFBPs) with various posttranslational modification are important for metabolism and distribution of IGFs, but their biological activity is independent from IGFs. In this study, one SNP mutation of G/A was rapidly screened by PCR with direct sequencing based on gene-pool in position +534 of IGFBP5 coding sequence, 300 F2 pigs of Jinhua×Pietrain crossbred was genotyped by PCR-RFLP. The results showed that three genotypes (GG, GA and AA) were found, the frequency of genotype GG, AG and AA were 0.064, 0.589 and 0.348 respectively, and the allele frequency of G and A was 0.358 and 0.642 respectively. The significant effect of various genotypes was found on weaning weight and water holding capacity by using the general linear model (p<0.05).
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Received: 21 October 2008
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