|
|
|
| Construction of plant expression vector of Hspa4 gene from Rattus norvegicus driven by maize ubi-1 promoter |
|
|
|
|
Abstract Hspa4 gene of Rattus norvegicus (Accession No.:NM 153629) and Ubi-1 promoter of maize (Accession No:DQ141598) were respectively cloned by RT-PCR and PCR technology. The sequencing and homologous analysis results showed that Hspa4 contains 2523bps and encodes 840 amino acids and the maize ubi-1 contains 1992bps. The cloned sequences have 99.96％ and 99.55% identity with their corresponding sequences published. Based on the plant expression vector pCAMBIA1301, we successfully construed pCAMBIA1301-Ubi-Hspa4, which would be useful for improving plant tolerance to environmental stresses.
|
|
Received: 10 September 2007
|
|
|
|
|
|
|
