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| Cloning and Development of Molecular Marker for the Wheat (Triticum aestivum) Cell Wall Invertase Gene TaCWINV41 |
| ZHANG Yu-Xuan1,2,*, CHU Yu-Hang1,2,*, XUE Bo-Yang1,2, MENG Heng-Yu1,2, ZHU Xue-Yuan1,2, WANG Guang-Liang1,2, LIU Bing-Liang1,2, ZHANG Rong-Zhi3, REN Yan4,5,**, LIU Jin-Dong6,**, FU Lu-Ping1,2,** |
1 Key Laboratory of Crop Genomics and Molecular Breeding of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Agricultural College, Yangzhou University, Yangzhou 225009, China;
2 Yangzhou University/Collaborative Innovation Center for Modern Industrial Technology of Grain Crops of Jiangsu Province, Yangzhou 225009, China;
3 Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China;
4 State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Beijing 100193, China;
5 College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China;
6 Institute of Crop Science, Chinese Academy of Agriculture Sciences, Beijing 100081, China |
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Abstract Grain filling rate (GFR) is a key factor influencing wheat (Triticum aestivum) thousand-kernel weight (TKW) and yield, while cell wall invertases (CWINVs) are core enzymes regulating plant carbohydrate metabolism and grain filling. It is of high significance to explore variations of wheat CWINV family genes and develop molecular markers for genetic improvement of wheat GFR and yield. In this study, a natural population comprising 166 wheat varieties (advanced lines) from the Yellow and Huai Valleys Winter Wheat Zone was used to measure the maximum grain filling rate (GFRmax) and TKW under 4 environments. With the genotyping data of wheat 660K and 90K SNP chips, genome-wide association study (GWAS) was conducted and a major-effect QTL simultaneously associated with GFRmax and TKW was identified at the 60.60~62.33 Mb interval on chromosome 4B, explaining 7.48%~12.40% and 7.53%~9.50% of the phenotypic variance for GFRmax and TKW, respectively. Referencing the 'Chinese Spring' reference genome, the TaCWINV41 gene (TraesCS4B02G066000) belonging to the CWINV family was located within the interval of this QTL. TaCWINV41 gene was cloned from cultivars 'Yangmai 16' and 'Zhongmai 895', and 2 SNPs were identified in its third exon; one of which, at position 1 892, was a C/T missense mutation. Based on this SNP, a kompetitive allele-specific PCR (KASP) marker, TaCWINV41_KASP, was developed. The reliability of this marker was validated using GFRmax and TKW phenotypic data of 194 wheat varieties. t-test analysis revealed that varieties with TT genotype exhibited significantly higher GFRmax and TKW than those with CC genotype (P<0.05, P<0.01, or P<0.001), confirming the value of TaCWINV41_KASP for molecular breeding. In summary, this study identified a major QTL simultaneously regulating wheat GFR and TKW through GWAS, uncovered allelic variation in the candidate gene TaCWINV41 at this locus, and developed a KASP marker for molecular breeding of wheat GFR and TKW. These findings provide theoretical insights into the genetic regulation of wheat GFR and offer an efficient molecular tool for genetic improvement of GFR and grain yield.
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Received: 14 January 2026
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Corresponding Authors:
**fuluping@yzu.edu.cn; liujindong@caas.cn; feier201000@163.com
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| About author:: *These authors contributed equally to this work |
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