花粉管通道遗传转化技术研究进展与应用

doi:10.3969/j.issn.1674-7968.2025.10.015

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Abstract The genetic transformation technology of pollen-tube pathway, as a commonly used method for genetic transformation, offers advantages such as simplicity of operation, independence from genotype, and the ability to directly obtain transgenic seeds. Despite being influenced by factors such as external environmental conditions, the natural flowering period of plants, and limited flowering frequency, the pollen tube pathway genetic transformation technique remains highly applicable to a wide range of plants, especially those that are genotype-dependent. This article reviewed the origin, classification, and characteristics of this method, discussed the main factors affecting its genetic transformation efficiency and the progress in understanding its transformation mechanisms, and compareed it with the Agrobacterium-mediated genetic transformation method. Finally, the current applications, existing issues, and future prospects of the pollen tube pathway method were elaborated, providing theoretical references for establishing or optimizing a rapid and highly efficient plant genetic transformation technique via the pollen tube pathway.

Key words： Plant genetic transformation Pollen-tube pathway Transformation efficiency Application prospect

Received: 13 March 2025

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Corresponding Authors: *changjiangyulu@163.com; 158066692@qq.com

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	GE Bao-Zhi
	SONG Jie
	PENG Lv-Chun
	LI Wei
	GUAN Wen-Ling
	ZHANG Lu

Cite this article:

GE Bao-Zhi,SONG Jie,PENG Lv-Chun, et al. Research Progress and Application of Genetic Transformation Technology by Pollen-tube Pathway[J]. 农业生物技术学报, 2025, 33(10): 2272-2287.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2025.10.015 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2025/V33/I10/2272

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