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| Study on Screening, Identification and Biological Characterization of Lignin-degrading Fungi |
| ZHANG Hao-Sen, LIU Tian-Qi, HU Xin-Miao, ZHANG Ri-Jun, SI Da-Yong* |
| College of Animal Science and Technology/Laboratory of Feed Biotechnology/State Key Laboratory of Animal Nutrition and Feeding, China Agricultural University, Beijing 100193, China |
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Abstract Lignocellulose, one of the primary components of agricultural waste straw, has a complex structure that limits the efficiency of cellulose degradation, resulting in a low utilization rate of straw resources. To enhance the efficient utilization of agricultural waste, this study isolated 17 fungal strains capable of growing on straw powder medium from soil, distiller's grains, and wild macrofungi samples in Yanbian Korean Autonomous Prefecture, Jilin Province. Through preliminary screening using guaiacol medium and secondary screening based on laccase activity, a highly efficient laccase-producing fungus, Coriolopsis trogii LFB-F1, was identified. Whole-genome sequencing and bioinformatics analysis revealed the genomic characteristics of this strain and the distribution of lignin-degrading genes. The results indicated that the LFB-F1 genome had a total length of 38.8 Mb, with a GC content of 54.93%, and contained 7 497 coding genes, of which 134 were carbohydrate-active enzymes (CAZymes), including 1 laccase gene and 5 lignin peroxidase genes. Biological characterization showed that LFB-F1 exhibited optimal growth at 30 ℃ and pH 5.5, with strong biomass accumulation capability. Enzymatic property analysis revealed that the optimal pH for LFB-F1 laccase activity was 4.5, with an optimal temperature of 45 ℃, and it exhibited good thermal stability at 35 ℃. Studies on the effects of metal ions on laccase activity showed that Cu2+ and Fe2+ had minimal impact, while Mn2+ strongly inhibited the enzyme. Additionally, the laccase retained 92.5% of its activity after 90 d of storage at 4 ℃, demonstrating excellent storage stability. In summary, the fungal strain isolated in this study exhibited lignin-degrading potential. The fungi screened in this study for the high efficiency in degrading lignin can provide theoretical and technical support for the resource utilization and high-value utilization of agricultural waste.
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Received: 10 March 2025
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Corresponding Authors:
*dayong@cau.edu.cn
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