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| Cloning and Activity Analysis of the HvSWEET4 Gene Promoter |
| YOU En1,2, XU Jin-Qing1,3, WANG Lei1,3, WANG Han-Dong1,3, CHEN Tong-Rui1,2, Tang You-Lin1,3, DENG Chao1,2, BIAN Hai-Yan1,2, LU Lu1,2, WANG Yi-Hang1,2, SHEN Yu-Hu1,3,* |
1 Key Laboratory of Adaptation and Evolution of Plateau Biota, Qinghai Provincial Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 Laboratory for Research and Utilization of Qinghai-Tibetan Plateau Germplasm Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China |
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Abstract HvSWEET4, a member of the sugars will eventually be exported transporters (SWEET) sugar transporter family in hulless barley (Hordeum vulgare var. nudum), is involved in hexose transport regulation during grain development. To elucidate the transcriptional regulatory mechanism of HvSWEET4, approximately 2 000 bp of promoter sequences in upstream of the coding region were systematically analyzed from 2 hulless barley cultivars with significantly different grain sizes: 'Beiqing 3' (large grain) and '3917' (small grain). Sequence comparison revealed that the HvSWEET4 promoters from these 2 cultivars were highly conserved, containing only 10 SNPs and 5 InDels, which accounted for merely 0.75% of the total nucleotides. Cis-element analysis indicated that the HvSWEET4 promoter contained regulatory elements associated with stress responses, sugar and starch metabolism and developmental regulation of pollen, endosperm and aleurone layers. Promoter activity assays identified the core functional region at -310~-212 bp. Further analyses suggested that differences in promoter activity could be attributed to suppressor effects within the region from -1 999~-310 bp, as well as SNP variations in the region from -212~-1 bp, potentially altering the types and sites of transcription factor binding. These findings provide essential theoretical foundations for further dissecting the molecular mechanisms underlying HvSWEET4 role in grain size regulation and highlight its potential applications in crop breeding.
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Received: 02 January 2025
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Corresponding Authors:
*shenyuhu@nwipb. cas. cn
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