MST1和LATS1在牦牛黄体形成、维持和退化中的表达及HCG对其表达的影响

doi:10.3969/j.issn.1674-7968.2024.08.012

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Abstract Mammalian STe20-like kinase 1 (MST1) and large tumor suppressor protein 1 (LATS1) are key members of the core kinase cascade of the Hippo pathway, which participate in the reproductive process of female animals and play an important role. This study aimed to explore the relationship between MST1, LATS1, and the formation, maintenance, and degeneration of yak (Bos grunniens) corpus luteum (CL). In this study, the corpus luteum of female yaks was collected and divided into 4 groups according to the different stages of corpus luteum formation (corpus rubrum stage, lacunose luteum stage), maintenance (pregnancy luteal phase) and degeneration (degenerative luteal phase). qPCR and Western blot were used to detect the differences of MST1, LATS1 mRNA and protein expression in the formation, maintenance, and degeneration of the corpus luteum. Immunohistochemistry (IHC) was used to analyze the localization of MST1 and LATS1 in the formation, maintenance, and degeneration of the corpus luteum. In addition, granulosa cells (GC) of the yak ovary were isolated and cultured, and luteinization of granulosa cells was induced by adding human chorionic gonadotropin (HCG). qPCR, Western blot and IF methods were used to analyze the quantity and localization of MST1, LATS1 mRNA and protein before and after luteinization. The results showed that MST1, LATS1 mRNA and protein were expressed in the yak corpus luteum, and there were differences in the expression of MST1 at different developmental stages of corpus luteum. The expression of MST1 was the highest in the pregnancy luteal phase, while the expression of LATS1 was the most abundant in the lacunose luteum stage, and the expression of MST1 and LATS1 was the lowest in the degenerative luteal phase. The results of IHC showed that MST1 and LATS1 were widely distributed in yak granular luteal cells, and MST1 was characterized by expression in vascular endothelial cells. After luteinization of granulosa cells induced by 5 IU/mL HCG, the expression of MST1, LATS1 mRNA and protein increased significantly. By IF staining, it was found that MST1 and LATS1 proteins were mainly located in the cytoplasm of granulosa cells, and the position did not change before and after luteinization. The results showed that MST1 and LATS1-mediated Hippo signaling pathway participates in the formation, maintenance, degeneration and functional operation of corpus luteum in female yaks by controlling granulosa cell differentiation, luteal cell proliferation and apoptosis, and which was closely related to various reproductive hormones in the physiological process of corpus luteum. This study provides basic data for further exploring the mechanism of the Hippo signal pathway regulating the size of the corpus luteum in yaks and regulating the reproductive physiological process of mammals.

Key words： Yak Corpus luteum Mammalian STE20-like kinase 1 (MST1) Large tumor suppressor kinase 1 (LATS1) Hippo pathway HCG

Received: 24 April 2023

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S857.2

Corresponding Authors: * fanjf@gsau.edu.cn

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	ZHOU Ying-Cong
	ZHANG Tong-Xiang
	WANG Jing-Lei
	ZHAO Ling
	ZHANG Rui
	YAO Ying
	QIAN Wen-Jie
	CUI Yan
	YU Si-Jiu
	FAN Jiang-Feng

Cite this article:

ZHOU Ying-Cong,ZHANG Tong-Xiang,WANG Jing-Lei, et al. The Expression of MST1 and LATS1 in the Formation, Maintenance and Degeneration of Corpus Luteum in Yak (Bos grunniens) and the Effect of HCG on Its Expression[J]. 农业生物技术学报, 2024, 32(8): 1844-1856.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2024.08.012 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2024/V32/I8/1844

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