西门塔尔牛9种遗传缺陷KASP检测方法的建立

doi:10.3969/j.issn.1674-7968.2023.11.020

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Abstract Simmental cattle (Bos taurus) and its related breeds are the main dual-purpose breeds and beef breeds in China. In recent years, multiple genetic defects, causing early embryonic death, newborn calf defects, reduced fertility and other problems and resulting in economic losses to farms, have been identified in Simmental cattle populations using genomic technologies. This study aimed to develop an accurate and efficient method based on kompetitive allele-specific PCR (KASP) assays to screen 9 genetic defects in Simmental cattle, including Dwarfism, Arachnomelia syndrome, Syndactyly, Thrombopathy, Zinc deficiency-like disorders, Bovine male subfertility, Brown Swiss haplotype 2, Fleckvieh haplotype 2 and Fleckvieh haplotype 4. The call rate was 100% for the 96 tested samples, and carriers were detected on 7 genetic defects. This study provides a technical tool for screening and management of harmful genes during the importation of genetic materials, selection of sires and mating programs of cows in China.

Key words： Kompetitive allele-specific PCR (KASP) Genetic defect Simmental Molecular assay

Received: 12 January 2023

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S823

Corresponding Authors: *yizhang@cau.edu.cn

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	Articles by authors
	PEI Fen
	ZHANG Ke
	KHAN Md Yousuf Ali
	SI Jing-Fang
	DAI Dong-Mei
	OU Si-Hai
	YANG Yang
	ZHAO Zong-Sheng
	WANG Ya-Chun
	ZHANG Yi

Cite this article:

PEI Fen,ZHANG Ke,KHAN Md Yousuf Ali, et al. Development of KASP Assays of 9 Genetic Defects in Simmental Cattle (Bos taurus)[J]. 农业生物技术学报, 2023, 31(11): 2435-2442.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2023.11.020 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2023/V31/I11/2435

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