|
|
Bioinformatics and Expression Analysis of StbZIP10 Gene Under Zinc Stress in Potato (Solanum tuberosum) |
LIU Hao-Tian1, ZHAO Jing2, TANG Xun1, ZHAO Gui-Bin3, ZHU Yong-Yong3, ZHANG Ning1, SI Huai-Jun1,* |
1 College of Life Science and Technology/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; 2 Gansu Agricultural Information Center, Lanzhou 730030, China; 3 Gansu General Station of Agro-technology Extension, Lanzhou 730020, China |
|
|
Abstract Zinc plays a role in many aspects of plant growth and development. However, excessive zinc and zinc deficiency can lead to physiological and biochemical disorders, forming zinc stress. Basic leucine zipper (bZIP) transcription factors play an important role in regulating plant growth and development and responding to stress. In this study, the full length of the potato (Solanum tuberosum) StbZIP10 gene (GenBank No. XM_006359636.1) was 1 653 bp with an CDS of 819 bp, encoding 272 amino acids. Bioinformatics analysis showed that StbZIP10 was a hydrophobic transmembrane protein with a relative molecular mass of 29.68 kD and had a typical bZIP conserved domain. The subcellular localization results showed that StbZIP10 protein was mainly expressed in the nucleus and cell membrane. The tissue-specific expression analysis of potato StbZIP10 gene was carried out by qRT-PCR. The results showed that the expression level of StbZIP10 gene was the highest in potato roots and the lowest in leaves, and there were significant difference among different plant tissues (P<0.05). The expression pattern of StbZIP10 in different plant tissues under zinc deficiency was the same, and the expression level in roots was the highest between 'Atlantic' and 'Longshu 7'. Under zinc deficiency treatment, the expression of StbZIP10 in both varieties was increased, but the expression of 'Longshu 7 ' was significantly higher than that of 'Atlantic' (P<0.05), and the trend was basically the same in the 3 time periods. The results could provide a theoretical basis for further elucidating the function of the potato StbZIP10 gene.
|
Received: 24 July 2022
|
|
Corresponding Authors:
*hjsi@gsau.edu.cn
|
|
|
|
[1] 杜平, 赵竹青, 宋波涛, 等. 2021. 马铃薯锌营养特性及锌生物强化技术研究进展[J]. 华中农业大学学报, 40(04): 36-43. (Du P, Zhao Z Q, Song B T, et al.2021. Advanc‐es on nutritional characteristics of zinc and zinc bioforti‐fication in potato[J]. Journal of Huazhong Agricultural University, 40(04): 36-43.) [2] 付学, 唐勋, 刘维刚, 等. 2020. 马铃薯StUBC12基因的生物信息学及表达特性分析[J]. 农业生物技术学报, 28(05): 784-793. (Fu X, Tang X, Liu WG, et al.2020. Bioinformatics and expression characteristics analysis of potato StUBC12 gene[J]. Journal of Agricultural Biotechnology, 28(05): 784-793.) [3] 胡焰, 韩光宇, 王健. 2011. 微量元素锌与人体健康初探[J]. 当代医学, 17(31): 152-153. (Hu Y, Han G Y, Wang J.2020. A preliminary study of trace element zinc and human health[J]. Contemporary Medicine, 28(05): 784-793.) [4] 王芳芳, 杨江伟, 朱熙, 等. 2021. 马铃薯StERF109基因的生物信息学及表达分析[J]. 农业生物技术学报, 29(11): 2087-2098. (Wang F F, Yang J W, Zhu X, et al.2021. Bioinformatics and expression analysis of potato StERF109 gene in Potato (Solanum tuberosum)[J]. Journal of Agricultural Biotechnology, 29(11): 2087-2098.) [5] 尹科, 赵婧, 唐勋, 等. 2022 .马铃薯锌转运体基因StZnT2生物信息学及表达特性分析[J]. 农业生物技术学报, 30(09): 1676-1686. (Yin K, Zhao Q, Tang X, et al.2022. Bioinformatics and expression analysis of potato (Solanum tuberosum) zinc transporter gene StZnT2[J]. Journal of Agricultural Biotechnology, 30(09): 1676-1686.) [6] Assunção A G, Herrero E, Lin Y F, et al.2010. Arabidopsis thaliana transcription factors bZIP19 and bZIP23 regulate the adaptation to zinc deficiency[J]. Proceedings of the National Academy of Sciences of the USA, 107(22): 10296-10301. [7] Birch P R J, Bryan G, Fento B, et al.2012. Crops that feed the world 8: Potato: Are the trends of increased global production sustainable?[J]. Food Security, 4: 477-508 [8] Bouain N, Krouk G, Lacombe B, et al.2019. Getting to the root of plant mineral nutrition: Combinatorial nutrient stresses reveal emergent properties[J]. Trends in Plant Science, 24(6): 542-552. [9] Cakmak I.2000. Possible roles of zinc in protecting plant cells from damage by reactive oxygen species[J]. New Phytologist, 146(2): 185-205. [10] Castro P H, Lilay G H, Muñoz-Mérida A, et al.2017. Phylogenetic analysis of F-bZIP transcription factors indicates conservation of the zinc deficiency response across land plants[J]. Scientific Reports, 7(1): 3806. [11] Devaux A, Goffart J P, Kromann P, et al.2021. The potato of the future: Opportunities and challenges in sustainable agri-food systems[J]. Potato Research, 64(4): 681-720. [12] Eide D J.2009. Homeostatic and adaptive responses to zinc deficiency in Saccharomyces cerevisiae[J]. Journal of Biological Chemistry, 284(28): 18565-9. [13] Gangadhar B H, Yu J W, Sajeesh K, et al.2014. A systematic exploration of high-temperature stress-responsive genes in potato using large-scale yeast functional screening[J]. Molecular Genetics and Genomics, 289(2): 185-201. [14] Gao M, Zhang H, Guo C, et al.2014. Evolutionary and expression analyses of basic zipper transcription factors in the highly homozygous model grape PN40024 (Vitis vinifera L.)[J]. Plant Molecular Biology Reporter, 32:1085-1102. [15] Glover-Cutter K M, Alderman S, Dombrowski J E, et al.2014. Enhanced oxidative stress resistance through activation of a zinc deficiency transcription factor in Brachypodium distachyon[J]. Plant Physiology, 166(3):1492-505. [16] Hambidge M.2000. Human zinc deficiency[J]. Journal of Nutrition, 130(5): 1344S-9S. [17] Hameed A, Zaidi S S, Shakir S, et al.2018. Applications of new breeding technologies for potato improvement[J]. Frontiers in Plant Science, 9: 925. [18] Heinekamp T, Strathmann A, Kuhlmann M, et al.2004. The tobacco bZIP transcription factor BZI-1 binds the GH3 promoter in vivo and modulates auxin-induced transcription[J]. Plant Journal, 38(2):298-309. [19] Inaba S, Kurata R, Kobayashi M, et al.2015. Identification of putative target genes of bZIP19, a transcription factor essential for Arabidopsis adaptation to Zn deficiency in roots[J]. Plant Journal, 84: 323-334. [20] Jia J, Zhao P, Cheng L, et al.2018. MADS-box family genes in sheepgrass and their involvement in abiotic stress responses[J]. BMC Plant Biology, 18(1): 42. [21] Landschulz W H, Johnson P F, McKnight S L.1988. The leucine zipper: A hypothetical structure common to a new class of DNA binding proteins[J]. Science, 240(4860): 1759-1764. [22] Liu T.2014. Use model-based analysis of ChIP-Seq (MACS) to analyze short reads generated by sequencing protein-DNA interactions in embryonic stem cells[J]. Methods in Molecular Biology, 1150: 81-95. [23] Lutaladio N B, Castaldi L.2009. Potato: The hidden treasure[J]. Journal of Food Composition & Analysis, 22(6):491-493. [24] Marc J, Bernd W, Wolfgang D, et al.2002. Bzip transcription factors in Arabidopsis[J]. Trends in Plant Science, 7(3): 106-111. [25] Marschner H.1995. Mineral nutrition of higher plants[J]. Annals of Botany, 78: 527-528. [26] Mikhaylina A, Ksibe A Z, Scanlan D J, et al.2018. Bacterial zinc uptake regulator proteins and their regulons[J]. Biochemical Society Transactions, 46(4): 983-1001. [27] Nazri A Z, Griffin J H C, Peaston K A, et al.2017. F-group bZIPs in barley-a role in Zn deficiency[J]. Plant, Cell and Environment, 40(11): 2754-2770. [28] Noulas C, Tziouvalekas M, Karyotis T.2018. Zinc in soils, water and food crops[J]. Journal of Trace Elements in Medicine and Biology, 49: 252-260. [29] Rao X Y, Huang X L, Zhou Z C, et al.2013. An improvementof the 2(-ΔΔCt) method for quantitative real-time polymerase chain reaction data analysis[J]. Biostatistics, Bioinformatics and Biomathematics, 3(3): 71-85. [30] Rizwan M, Ali S, Rehman M Z U, et al.2019. A critical review on the effects of zinc at toxic levels of cadmium in plants[J]. Environmental Science and Pollution Research, 26(7): 6279-6289. [31] Rook F, Weisbeek P, Smeekens S.1998. The light-regulated Arabidopsis bZIP transcription factor gene ATB2 encodes a protein with an unusually long leucine zipper domain[J]. Plant Molecular Biology, 37(1): 171-178. [32] Strathmann A, Kuhlmann M, Heinekamp T, et al.2001. BZI-1 specifically heterodimerises with the tobacco bZIP transcription factors BZI-2, BZI-3/TBZF and BZI-4, and is functionally involved in flower development[J]. Plant Journal, 28(4): 397-408. [33] Yang O, Popova O V, Süthoff U, et al.2009. The Arabidopsis basic leucine zipper transcription factor AtbZIP24 regulates complex transcriptional networks involved in abiotic stress resistance[J]. Gene, 436(1-2): 45-55. [34] Yang X E, Chen W R, Feng Y.2007. Improving human micronutrient nutrition through biofortification in the soil-plant system: China as a case study[J]. Environmental Geochemistry and Health, 29(5): 413-428. [35] Zou M, Guan Y, Ren H, et al.2008. A bZIP transcription factor, OsABI5, is involved in rice fertility and stress tolerance[J]. Plant Molecular Biology, 66(6): 675-683. |
|
|
|