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| The Genetic Effect Analysis of the Major QTL qTkw-2D for Grain Weight in Wheat (Triticum aestivum) |
| ZHANG Na1, ZHANG Shuai2, ZHI Li-Ya2, SONG Li-Qiang2, LIU Dong-Tao1, MA Hong-Bo1, JI Jun2,* |
1 Jiangsu Xuhuai Regional Institute of Agricultural Sciences, Xuzhou 221131, China;
2 Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050021, China |
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Abstract In wheat (Triticum aestivum), grain weight selecting is effective for improvement of grain yield as its high heredity and stable phenotype. In previous study, a stable QTL for grain weight, qTkw-2D, was identified and this QTL has been verified in several populations. In this study, the linkage marker Xcfd233 and Wheat660K SNP array were used to scan 3 000 high generation of breeding population. Finally, near isogenic lines (NILs) of qTkw-2D were achieved in the F6 generation of 'Kenong 2011'. By comparison the pericarp cells size of the grain slices from two NILs, it was confirmed that qTkw-2D mainly affected grain width by affecting the size of inner pericarp cells. The analysis results of filling rate showed that qTkw-2D mainly affected grain weight by affecting the grain filling rate during middle and late filling stage. The grain weight in different environments at individual and population level of NILs indicated that qTkw-2D influenced grain yield in both levels and haplotype A was of great importance to maintain grain yield during poor harvest environments. This study provides reference for grain weight improvement and function gene cloning.
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Received: 10 May 2023
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Corresponding Authors:
*jijun@sjziam.ac.cn
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[1] Alexander W L, Smith E L, Dhanasobhan C A.1984. Comparison of yield and yield component selection in winter wheat[J]. Euphytica, 33(3): 953-961.
[2] Cui F, Fan X, Chen M, et al.2016. QTL detection for wheat kernel size and quality and the responses of these traits to low nitrogen stress[J]. Theoretical and Applied Genetics, 129(3): 469-484.
[3] Jiang Q, Hou J, Hao C, et al.2011. The wheat (Triticum aestivum L.) sucrose synthase 2 gene (TaSus2) active in endosperm development is associated with yield traits[J]. Functional & Integrative Genomics, 11(1): 49-61.
[4] Liu H, Li H, Hao C, et al.2020. TaDA1, a conserved negative regulator of kernel size, has an additive effect with TaGW2 in common wheat (Triticum aestivum L.)[J]. Plant Biotechnology Journal, 18(5): 1330-1342.
[5] Ma L, Li T, Hao C, et al.2016. TaGS5‐3A, a grain size gene selected during wheat improvement for larger kernel and yield[J]. Plant Biotechnology Journal, 14(5): 1269-1280.
[6] Ma M, Wang Q, Li Z, et al.2015. Expression of TaCYP78A3, a gene encoding cytochrome P450 CYP78A3 protein in wheat (Triticum aestivum L.), affects seed size[J]. Plant Journal. 83:312-325.
[7] Pang Y, Liu C, Wang D, et al.2020. High-resolution genome-wide association study identifies genomic regions and candidate genes for important agronomic traits in wheat[J]. Molecular Plant, 13(9): 1311-1327.
[8] Qin L, Hao C, Hou J, et al.2017. Homologous haplotypes, expression, genetic effects and geographic distribution of the wheat yield gene TaGW2[J]. BMC Plant Biology, 14: 107.
[9] Ramya P, Chaubal A, Kulkarni K, et al.2010. QTL mapping of 1000-kernel weight, kernel length, and kernel width in bread wheat (Triticum aestivum L.)[J]. Journal of Applied Genetics, 51(4): 421-429.
[10] Simmonds J, Scott P, Brinton J, et al.2016. A splice acceptor site mutation in TaGW2-A1 increases thousand grain weight in tetraploid and hexaploid wheat through wider and longer grains[J]. Theoretical and Applied Genetics, 129: 1099-1112.
[11] Su Q, Zhang X, Zhang W, et al.2018. QTL detection for kernel size and weight in bread wheat (Triticum aestivum L.) using a high-density SNP and SSR-based linkage map[J]. Frontiers in Plant Science, 9: 1484.
[12] Su Z, Hao C, Wang L, Dong, Yet al.2011. Identification and development of a functional marker of TaGW2 associated with grain weight in bread wheat (Triticum aestivum L.)[J]. Theoretical and Applied Genetics, 122(1): 211-223.
[13] Sun C, Zhang F, Yan X, et al.2017. Genome-wide association study for 13 agronomic traits reveals distribution of superior alleles in bread wheat from the Yellow and Huai Valley of China[J]. Plant Biotechnology Journal, 15(8): 953-969.
[14] Wang L F, Ge H M, Hao C Y, et al.2012. Identifying loci influencing 1,000-kernel weight in wheat by microsatellite screening for evidence of selection during breeding[J]. PLOS ONE, 7(2): e0029432.
[15] Wang S, Zhang X, Chen F, et al.2015. A single-nucleotide polymorphism of TaGS5 gene revealed its association with kernel weight in Chinese bread wheat[J]. Frontiers in Plant Science, 6: 1166.
[16] Wang Y, Hou J, Liu H, et al.2019. TaBT1, affecting starch synthesis and thousand kernel weight, underwent strong selection during wheat improvement[J]. Journal of Experimental Botany, 70(5): 1497-1511.
[17] Wu X, Liu J, Li D, et al.2016. Rice caryopsis development Ⅱ: Dynamic changes in the endosperm[J]. Journal of Integrative Plant Biology, 58: 786-798.
[18] Yang J, Zhou Y, Zhang Y, et al.2019. Cloning, characterization of TaGS3 and identification of allelic variation associated with kernel traits in wheat (Triticum aestivum L.)[J]. BMC Genetics, 20(1): 98.
[19] Zhang D L, Hao C Y, Wang L F, et al.2012. Identifying loci influencing grain number by microsatellite screening in bread wheat (Triticum aestivum L.)[J]. Planta, 236(5): 1507-1517.
[20] Zhang G Z, Wang Y Y, Guo Y, et al.2015. Characterization and mapping of QTLs on chromosome 2D for grain size and yield traits using a mutant line induced by EMS in wheat[J]. Crop Journal, 3(2): 135-144.
[21] Zhang L, Zhao Y L, Gao L F, et al.2012. TaCKX6D1, the ortholog of rice OsCKX2, is associated with grain weight in hexaploid wheat[J]. New Phytologist, 195(3): 574-584.
[22] Zhang N, Zhang X L, Song L Q, et al.2020. Identification and validation of the superior alleles for wheat kernel traits detected by genome-wide association study under different nitrogen environments[J]. Euphytica, 216: 52.
[23] Zhang W, Li H F, Zhi L Y, et al.2020. Functional markers developed from TaGS3, a negative regulator of grain weight and size, for marker-assisted selection in wheat[J]. Crop Journal, 8(6): 943-952.
[24] Zhang Y, Liu J, Xia X, et al.2014. TaGS-D1, an ortholog of rice OsGS3, is associated with grain weight and grain length in common wheat[J]. Molecular Breeding, 34(3): 1097-1107.
[25] Zuo J, Li J.2014. Molecular genetic dissection of quantitative trait loci regulating rice grain size[J]. Annual Review of Genetics, 48: 99-118. |
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