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| Identification of SPX Gene Family in Phoebe bournei and Its Expression Analysis Under Phosphorus Deficiency Stress |
| WU Meng-Jie1, HONG Jia-Du1, LI Fang-Yan1, ZHOU Sheng-Cai2, CHENG Long-Jun1,* |
1 College of Forestry and Biotechnology/State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China;
2 Lishui Vocational & Technical College, Lishui 323000, China; |
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Abstract Phoebe bournei is a precious tree spiece unique to China. Afforestation in red loam which is very popular in south of China is prone to low phosphorus stress, affecting cultivation benefit. Exploring the gene resources in P. bournei with phosphorus tolerance is of great significance to develop P. bournei varieties with enhancing ability of low phosphorus tolerance by molecular assisted breeding. SPX gene is associated with phosphorus absorption and balance in plants. In this study, members of the SPX gene family in P. bournei were identified in the whole genome, and the expression of PbSPX gene in the roots, stems, and leaves of P. bournei were analyzed using qPCR, as well as the expression patterns in the roots and leaves of P. bournei after 10 and 60 d of phosphorus deficiency treatment. The results showed that 20 PbSPXs were distributed on chromosomes 1 to 10 of P. bournei. According to characteristics of protein and phylogenetic tree, PbSPXs were divided into 4 subfamilies, including 6 members of SPX subfamily, 7 members of SPX-EXS subfamily, 3 members of SPX-MFS subfamily and 4 members of SPX-RING subfamily. Analysis of promoter cis-acting elements showed that PbSPX genes containing elements related to phosphorus stress response, such as P1BS, W-box and MBS. Tissue specific expression analysis showed that PbSPX2, PbSPX-EXS1 and PbSPX-RING2 were mainly expressed in roots, and another 12 PbSPXs were expressed highly in leaves. In roots, when treated with phosphorus deficiency after 10 and 60 d, the expression of PbSPX-MFS1 and all PbSPX subfamily members except for PbSPX6 were induced and the expression of them were significantly up-regulated compared to the control, however, the expression of PbSPX-MFS2 was significantly down-regulated. At the same time, the transcript levels of PbSPX-RING1 and PbSPX-EXS1 were significantly increased at 10 d of phosphorus deficiency treatment, while the expressions of PbSPX-RING3 and PbSPX-EXS2 were significantly up-regulated until after 60 d of phosphorus deficiency treatment. The expression of PbSPX-EXS6 was significantly decreased when treated with phosphorus deficient for 10 d, whereas it was significantly up-regulated after 60 d of phosphorus deficiency treatment. In leaves, the expressions of PbSPX1~5, PbSPX-EXS1 and PbSPX-MFS1 were induced after 60 d of phosphorus deficiency treatments, and their expressions were significantly up-regulated compared to the control. The transcript level of PbSPX-MFS2 was significantly down-regulated after 60 d of phosphorus deficiency treatment, which was consistent with the expression trend in roots. As phosphorus transporters candidates, PbSPX-EXS1, PbSPX-EXS2 and PbSPX-EXS6 responded differently to phosphorus level signals in different tissues. The results showed that members of the SPX subfamily of P. bournei were involved in phosphorus stress response, and their expression was regulated by phosphorus levels in different tissues. This study revealed the relationship between PbSPX genes and the response of low phosphorus stress in P. bournei, providing a reference for exploiting key gene resources of low phosphorus tolerance in P. bournei and further studying function and mechanism of PbSPX.
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Received: 22 September 2022
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Corresponding Authors:
* ljcheng@zju.edu.cn
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