Clone and Expression Analysis of Regulator of Glycerol Channel 2 Gene (StRGC2) in Setosphaeria turcica
SUN Ming-Xuan1, LI Zheng-Zheng1, LIU Yu-Wei1, GONG Xiao-Dong1, FAN Yong-Shan2, HAN Jian-Min1, GU Shou-Qin1,*, DONG Jin-Gao1,*
1 College of Life Sciences/Key Laboratory of Plant Physiology and Molecular Pathology of Hebei Province/State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, China; 2 Department of Life Sciences, Tangshan Normal University, Tangshan 063002, China
Abstract Regulator of glycerol channel 2 (RGC2) is a key factor which is down stream in high osmolarity glycerol-mitogen-activated protein kinase (HOG-MAPK) cascade pathway involved in regulation of hyperosmotic stress reaction. In Saccharomyces cerevisiae, ScRGC2 was found to be involved in regulating the concentration of glycerol in the cells and appropriate glycerol content can help cells protect from outside damage. To identify structure characteristic and expression pattern of StRGC2 during its key growth and development periods and under hypertonic stress in Setosphaeria turcica, StRGC2 (GenBank No. XP_008024338) was cloned. The structural analysis based on the gene and its coding protein showed that the gene contained 4 exons and 3 introns, the full length of DNA was 1 857 bp and the full length of cDNA was 1 389 bp. The C-terminal of its coded protein contained a PH conserved domain and was located in the nucleus. The expression patterns of StRGC2 gene at different developmental stages (hypha, conidia, germ tube, invasive nail, appressorium formation) were analyzed in virtue of RNA-seq data obtained in this previous research and the highest expression level appeared in appressorium development stage. Prediction of the promoter element showed that there were various abiotic stress-induced element. Among them, stress-induced element related to hypertonic stress were found. Further, the expression pattern of StRGC2 gene under hypertonic stress showed that the expression level of StRGC2 gene increased sharply under 0.4 mol/L NaCl for 3 h compared to the control, meaning StRGC2 involved in the regulation of hypertonic stress response in S. turcica. This study not only clarified the structural characteristics and expression pattern of StRGC2 gene, but also laid foundation for the function research of StRGC2 gene and its molecular mechanism in the pathogenic process of S. turcica.
SUN Ming-Xuan,LI Zheng-Zheng,LIU Yu-Wei, et al. Clone and Expression Analysis of Regulator of Glycerol Channel 2 Gene (StRGC2) in Setosphaeria turcica[J]. 农业生物技术学报, 2023, 31(3): 594-602.
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